The women were charged after a Metropolitan Police inquiry into Whipps Cross hospital in north London.
Akousa Sakyiwaa, 37, of Orange Grove, Leytonstone, and Annette Jackson, 32, of Simpson Road, Hounslow, face several counts of ill treatment or neglect under the Mental Capacity Act 2005.
Ms Sakyiwaa was also charged with common assault.
Sharmilla Gunda, 35, of Horns Road, Ilford, was charged with one count of the same offence and one count of fraud in relation to alleged falsifying of records of the number of hours worked.
They will appear on bail on Thames magistrates court on October 31, police said.