The veteran sprinter, who has struggled with a back problem which forced him to miss last month's trials, lacked his customary power at the Scandinavium Arena as he came home fifth in 6.78 seconds.
That was way down on his best this season of 6.58secs and not even quick enough for a fastest loser spot.
A visibly furious Chambers, the defending silver medallist, stormed through the mixed zone afterwards, not stopping to talk to the media, having already angrily discarded his shirt and shoes.