City go into the final day clash at Eastlands level on points with Manchester United but with a superior goal difference that is unlikely to be overturned.
United go to mid-table Sunderland while City face a QPR side who need a point to all but guarantee their survival in the Premier League.
And Mancini feels playing a team who have so much at stake puts them at a disadvantage.
He said: “When you play the last game and, if you win, you can win the title — it is a big game. In this situation we are playing a team fighting against relegation. It will be hard, more difficult than (previous opponents) Newcastle, more than United.
“Sunderland play for nothing. I am sure they will play 100 per cent, but they play for nothing in their last game.”
The battle for Champions League qualification has also come down to the final day.
Arsenal in third visit West Brom, fourth-placed Tottenham host Fulham while Newcastle in fifth take on Everton at Goodison Park.
The Gunners are a point ahead of Spurs with Newcastle a further point adrift. Third place guarantees a place in Europe’s elite club competition and the fourth-placed team would also qualify unless Chelsea beat Bayern Munich in the final of this year’s tournament next weekend.